Hotspot

petB-petD

LSC · 77212-78139 · 928 bp

Back to Primulina

Pi 0.0177
MarkerSeek score 82.70
Primer available yes
Label petB-petD

Feature Metadata

label_name
petB-petD
feature_type
igs
region
LSC
length_bp
928
variable_sites
79
pi
0.0177
unique_haplotype_count
22
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB-petD_p1 ACTTTTGTACTGCCGCTTCT TGACCCATACCTTTTGCCAA 45.0 45.0 57.7 57.3 1109-1141 bp 1.0 81.5
petB-petD_p2 CACTTTTGTACTGCCGCTTC TGACCCATACCTTTTGCCAA 50.0 45.0 57.7 57.3 1110-1142 bp 1.0 81.0
petB-petD_p3 ACACTTTTGTACTGCCGCTT TGACCCATACCTTTTGCCAA 45.0 45.0 58.3 57.3 1111-1143 bp 1.0 80.9
petB-petD_p4 TGTACTGCCGCTTCTTACTG TGACCCATACCTTTTGCCAA 50.0 45.0 57.6 57.3 1104-1136 bp 1.0 80.7
petB-petD_p5 GTACTGCCGCTTCTTACTGT TGACCCATACCTTTTGCCAA 50.0 45.0 57.6 57.3 1103-1135 bp 1.0 80.7