Hotspot

atpF-atpH

LSC · 13463-13849 · 387 bp

Back to Primulina

Pi 0.0171

MarkerSeek score 74.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 387
variable_sites: 34
pi: 0.0171
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGGCCTAAAGAAACGA	AGGCAGAGGGAAAGATACGA	50.0	50.0	57.8	57.8	493-545 bp	1.0	87.3
atpF-atpH_p2	TTAAATCCGAAACTCCCGGC	AGGCAGAGGGAAAGATACGA	50.0	50.0	58.3	57.8	519-571 bp	1.0	86.4
atpF-atpH_p3	CCAGTGGCCTAAAGAAACGA	GCAGAGGGAAAGATACGAGG	50.0	55.0	57.8	57.5	491-543 bp	1.0	84.5
atpF-atpH_p4	CCAGTGGCCTAAAGAAACGA	GGCAGAGGGAAAGATACGAG	50.0	55.0	57.8	57.5	492-544 bp	1.0	84.5
atpF-atpH_p5	CCAGTGGCCTAAAGAAACGA	GAGGCAGAGGGAAAGATACG	50.0	55.0	57.8	57.5	494-546 bp	1.0	84.4