Hotspot

rps16-trnQ-UUG

LSC · 6220-7283 · 1064 bp

Back to Primulina

Pi 0.0203
MarkerSeek score 68.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1064
variable_sites
117
pi
0.0203
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGATGTCGAGACAAGAGC AGCAGCTTGCCAAACAAAAG 50.0 45.0 58.0 58.1 1285-1855 bp 1.0 90.9
rps16-trnQ-UUG_p2 AACGATGTCGAGACAAGAGC CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.0 57.9 1290-1860 bp 1.0 90.9
rps16-trnQ-UUG_p3 AACGGATCGTGTCCTTCAAG AGCAGCTTGCCAAACAAAAG 50.0 45.0 57.9 58.1 1214-1784 bp 0.954545 88.2
rps16-trnQ-UUG_p4 AACGGATCGTGTCCTTCAAG CTTACAGCAGCTTGCCAAAC 50.0 50.0 57.9 57.9 1219-1789 bp 0.954545 88.1
rps16-trnQ-UUG_p5 AACGATGTCGAGACAAGAGC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1042-1247 bp 0.409091 61.3