Hotspot

psbA-trnK-UUU

LSC · 1470-1707 · 238 bp

Back to Primulina

Pi 0.0189

MarkerSeek score 72.90

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 238
variable_sites: 32
pi: 0.0189
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TACAGAAGCGACCCCATAGG	CTAGTTCCGGGTTCGAATCC	55.0	55.0	58.6	57.8	314-346 bp	1.0	85.5
psbA-trnK-UUU_p2	ATCCAGTTACAGAAGCGACC	CTAGTTCCGGGTTCGAATCC	50.0	55.0	57.3	57.8	321-353 bp	0.954545	82.0
psbA-trnK-UUU_p3	AGTTACAGAAGCGACCCCAT	CTAGTTCCGGGTTCGAATCC	50.0	55.0	58.7	57.8	317-349 bp	0.954545	81.7
psbA-trnK-UUU_p4	AGACGGTTTTCAGTGTTGGT	CTAGTTCCGGGTTCGAATCC	45.0	55.0	57.6	57.8	352 bp	0.045455	36.7
psbA-trnK-UUU_p5	AGACGGTTTTCAGTGTTGGT	ATGAAAAATGCAAGCACGGC	45.0	45.0	57.6	58.6	432 bp	0.045455	34.0