Hotspot

trnH-GUG-psbA

LSC · 79-410 · 332 bp

Back to Primulina

Pi 0.0657

MarkerSeek score 76.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 332
variable_sites: 79
pi: 0.0657
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCGGTCAGGAGGAAGAAACT	55.0	50.0	58.1	58.0	647-773 bp	1.0	90.9
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TCGGTCAGGAGGAAGAAACT	50.0	50.0	57.9	58.0	661-787 bp	1.0	90.8
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TCGGTCAGGAGGAAGAAACT	50.0	50.0	57.9	58.0	660-786 bp	1.0	90.8
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TCGGTCAGGAGGAAGAAACT	50.0	50.0	57.9	58.0	662-788 bp	1.0	90.8
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	GTTATACAACGGCGGTCCTT	55.0	50.0	58.1	58.0	1048-1174 bp	1.0	90.4