Hotspot

matK

LSC · 2079-3611 · 1533 bp

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Pi 0.0524

MarkerSeek score 84.50

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1533
variable_sites: 549
pi: 0.0524
unique_haplotype_count: 102
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	AGCAGCAATGAAGGCGATAA	AAGACCACGACTGATCCTGA	45.0	50.0	58.0	58.1	2824-2892 bp	0.951456	89.1
matK_p2	AGCAGCAATGAAGGCGATAA	AGACCACGACTGATCCTGAA	45.0	50.0	58.0	58.1	2823-2891 bp	0.932039	88.3
matK_p3	ACTCCCAAGCACACGAATTT	AAGACCACGACTGATCCTGA	45.0	50.0	58.0	58.1	2633-2692 bp	0.533981	71.9
matK_p4	ACTCCCAAGCACACGAATTT	AGACCACGACTGATCCTGAA	45.0	50.0	58.0	58.1	2632-2691 bp	0.533981	71.9
matK_p5	ACTCCCAAGCACACGAATTT	GTGTGGGTGCGAGAAATACT	45.0	50.0	58.0	57.9	2360-2415 bp	0.349515	63.6