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petA-psbJ

LSC · 62854-63864 · 1011 bp

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Pi 0.0738

MarkerSeek score 83.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1011
variable_sites: 457
pi: 0.0738
unique_haplotype_count: 101
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTTGTGATAGGCCCTGTTCC	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.0	58.0	1637-2227 bp	0.932039	88.9
petA-psbJ_p2	CTTGTGATAGGCCCTGTTCC	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.0	58.0	1636-2226 bp	0.932039	88.9
petA-psbJ_p3	TCAGAGCTATCGCCCCAATA	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	1692-2282 bp	0.92233	88.5
petA-psbJ_p4	TCAGAGCTATCGCCCCAATA	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1668-2258 bp	0.873786	86.8
petA-psbJ_p5	TCAGAGCTATCGCCCCAATA	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1667-2257 bp	0.873786	86.8