Hotspot

rbcL-accD

LSC · 56588-57207 · 620 bp

Back to Primula

Pi 0.0526

MarkerSeek score 84.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 620
variable_sites: 248
pi: 0.0526
unique_haplotype_count: 100
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	ATTGAACTGCCATTTTTCCA	50.0	35.0	57.9	53.9	576-740 bp	0.932039	55.7
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	TTGAACTGCCATTTTTCCAT	50.0	35.0	57.9	53.9	575-739 bp	0.92233	55.3
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	AATTGAACTGCCATTTTTCCA	50.0	33.3	57.9	54.8	577-741 bp	0.932039	54.7
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	ATTGAACTGCCATTTTTCCAT	50.0	33.3	57.9	54.6	576-740 bp	0.92233	52.5
rbcL-accD_p5	CTGCTGCTTGTGAGGTATGG	TTGAACTGCCATTTTTCCAT	55.0	35.0	58.6	53.9	577-741 bp	0.893204	50.0