Hotspot

trnF-GAA-ndhJ

LSC · 48055-48636 · 582 bp

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Pi 0.0701

MarkerSeek score 82.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 582
variable_sites: 277
pi: 0.0701
unique_haplotype_count: 101
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGAAATACAGGATGCTCATTGA	55.0	36.4	58.0	55.5	203-789 bp	0.990291	55.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TGAAATACAGGATGCTCATTGA	50.0	36.4	58.1	55.5	167-753 bp	0.980583	54.9
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	TGAAATACAGGATGCTCATTGA	55.0	36.4	57.9	55.5	191-777 bp	0.980583	54.2
trnF-GAA-ndhJ_p4	AATCCTCGTGTCACCAGTTC	TGAAATACAGGATGCTCATTGA	50.0	36.4	57.5	55.5	170-756 bp	0.990291	52.0
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	ATGAAATACAGGATGCTCATTGA	55.0	34.8	58.0	56.0	204-790 bp	0.990291	51.9