Hotspot

trnT-UGU-trnL-UAA

LSC · 46248-47042 · 795 bp

Back to Primula

Pi 0.06

MarkerSeek score 82.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 795
variable_sites: 351
pi: 0.06
unique_haplotype_count: 101
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	AAAACCTGACCTGTTGACCC	ACCAATTTCGCCATATCCCC	50.0	50.0	57.9	58.0	1345-1575 bp	0.961165	89.9
trnT-UGU-trnL-UAA_p2	AAAACCTGACCTGTTGACCC	AAGTCGACGGATTTTCCTCG	50.0	50.0	57.9	58.0	1883-2032 bp	0.38835	66.9
trnT-UGU-trnL-UAA_p3	AACTAATTGGCGAGCTCCAG	ACCAATTTCGCCATATCCCC	50.0	50.0	58.0	58.0	1559 bp	0.009709	40.1
trnT-UGU-trnL-UAA_p4	AACTAATTGGCGAGCTCCAG	AAGTCGACGGATTTTCCTCG	50.0	50.0	58.0	58.0	2093 bp	0.009709	40.1
trnT-UGU-trnL-UAA_p5	AACTAATTGGCGAGCTCCAG	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.0	58.1	1567 bp	0.009709	39.6