Hotspot

pafI-trnS-GGA

LSC · 43995-44853 · 859 bp

Back to Primula

Pi 0.0658

MarkerSeek score 82.90

Primer available yes

Label pafI-trnS-GGA

Feature Metadata

label_name: pafI-trnS-GGA
feature_type: igs
region: LSC
length_bp: 859
variable_sites: 369
pi: 0.0658
unique_haplotype_count: 100
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-trnS-GGA_p1	TTCTTCCTCTATGCCGCCTA	CGTTCGGGCAATTTTGGTAC	50.0	50.0	57.9	58.0	1388-1653 bp	0.495146	70.9
pafI-trnS-GGA_p2	GCCGCCTAGTGGTACTAGTA	CTGGAGCTCGCCAATTAGTT	55.0	50.0	58.0	58.0	1688-1953 bp	0.368932	66.3
pafI-trnS-GGA_p3	GCCGCCTAGTGGTACTAGTA	CCCATTCAGGAAAGGTACGG	55.0	55.0	58.0	58.0	1122-1388 bp	0.368932	66.2
pafI-trnS-GGA_p4	GCCGCCTAGTGGTACTAGTA	CGTTCGGGCAATTTTGGTAC	55.0	50.0	58.0	58.0	1376-1641 bp	0.330097	64.8
pafI-trnS-GGA_p5	GCCGCCTAGTGGTACTAGTA	ATCGTTGCAAACCCCAAGAT	55.0	45.0	58.0	58.1	1860 bp	0.009709	39.6