Hotspot

trnT-GGU-psbD

LSC · 31358-32621 · 1264 bp

Back to Primula

Pi 0.0621

MarkerSeek score 84.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1264
variable_sites: 564
pi: 0.0621
unique_haplotype_count: 102
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	AAGAAATTGCAGCCCTCCAA	50.0	45.0	57.9	58.0	1177-1616 bp	0.951456	89.1
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	AGAAATTGCAGCCCTCCAAA	50.0	45.0	57.9	58.0	1176-1615 bp	0.951456	89.1
trnT-GGU-psbD_p3	TAGACGATGGGGGCATACTT	AAGAAATTGCAGCCCTCCAA	50.0	45.0	57.9	58.0	1741-2457 bp	0.883495	86.5
trnT-GGU-psbD_p4	TAGACGATGGGGGCATACTT	AGAAATTGCAGCCCTCCAAA	50.0	45.0	57.9	58.0	1740-2456 bp	0.883495	86.5
trnT-GGU-psbD_p5	TAGACGATGGGGGCATACTT	ACAAAAACAGCAATTGGGCC	50.0	45.0	57.9	58.0	1981-2697 bp	0.747573	80.9