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psbM-trnD-GUC

LSC · 29137-30248 · 1112 bp

Back to Primula

Pi 0.0775

MarkerSeek score 81.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1112
variable_sites: 505
pi: 0.0775
unique_haplotype_count: 100
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATTTACCGCTTACCGCTTGT	AAGTATGCCCCCATCGTCTA	45.0	50.0	57.9	57.9	2076-2229 bp	0.048544	47.1
psbM-trnD-GUC_p2	TACCGCTTACCGCTTGTTTT	AAGTATGCCCCCATCGTCTA	45.0	50.0	58.1	57.9	2073-2226 bp	0.048544	47.0
psbM-trnD-GUC_p3	TTACCGCTTACCGCTTGTTT	AAGTATGCCCCCATCGTCTA	45.0	50.0	58.1	57.9	2074-2227 bp	0.048544	47.0
psbM-trnD-GUC_p4	TTTACCGCTTACCGCTTGTT	AAGTATGCCCCCATCGTCTA	45.0	50.0	58.1	57.9	2075-2228 bp	0.048544	47.0
psbM-trnD-GUC_p5	ATTTACCGCTTACCGCTTGT	TGGATTCTTCCTGGGTCGAT	45.0	50.0	57.9	58.1	1945-2098 bp	0.048544	47.0