Hotspot

ndhF

SSC · 111938-114202 · 2265 bp

Pi 0.00682

MarkerSeek score 48.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2265
variable_sites: 26
pi: 0.00682
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	AAAGAGGTAAGGTCCGGTGA	50.0	50.0	58.0	58.0	2507-2615 bp	1.0	83.8
ndhF_p2	ACAGGAACAAGAGGGATCCA	AAGAGGTAAGGTCCGGTGAA	50.0	50.0	58.0	58.0	2506-2614 bp	1.0	83.8
ndhF_p3	AAGAGGGATCCACCGAAGAA	AAAGAGGTAAGGTCCGGTGA	50.0	50.0	58.0	58.0	2499-2607 bp	1.0	83.8
ndhF_p4	AAGAGGGATCCACCGAAGAA	AAGAGGTAAGGTCCGGTGAA	50.0	50.0	58.0	58.0	2498-2606 bp	1.0	83.8
ndhF_p5	CCGAAGAAGATCCTCCTCCT	AAGAGGTAAGGTCCGGTGAA	55.0	50.0	57.9	58.0	2486-2594 bp	1.0	83.6