Hotspot

ccsA

SSC · 115541-116518 · 978 bp

Pi 0.00341

MarkerSeek score 46.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 6
pi: 0.00341
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGGTATGAGGCAGCGTTAAA	TGAATTGGAACTGGCGAGAG	50.0	50.0	58.0	57.9	1858-1900 bp	1.0	82.4
ccsA_p2	ATTCCCGAATTCCATTCCCG	TGAATTGGAACTGGCGAGAG	50.0	50.0	58.0	57.9	1688-1730 bp	0.5	61.0
ccsA_p3	CCCGAATTCCATTCCCGATT	TGAATTGGAACTGGCGAGAG	50.0	50.0	58.0	57.9	1685-1727 bp	0.5	61.0
ccsA_p4	CCCGAATTCCATTCCCGATT	TTTTTGATTCTGGACCGCGA	50.0	45.0	58.0	58.1	1943-1980 bp	0.5	60.9
ccsA_p5	ATTCCCGAATTCCATTCCCG	TTTTTGATTCTGGACCGCGA	50.0	45.0	58.0	58.1	1946-1983 bp	0.5	60.8