Hotspot

ndhE-ndhG

SSC · 119235-119466 · 232 bp

Back to Premna

Pi 0.00845
MarkerSeek score 61.70
Primer available yes
Label ndhE-ndhG

Feature Metadata

label_name
ndhE-ndhG
feature_type
igs
region
SSC
length_bp
232
variable_sites
3
pi
0.00845
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.93
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhG_p1 AGACACATAAGAGCTCTAACCA TCGCTTTAATAGGTGCTATTGC 40.9 40.9 56.3 56.9 355-371 bp 1.0 44.2
ndhE-ndhG_p2 AGACACATAAGAGCTCTAACCA CGCTTTAATAGGTGCTATTGCT 40.9 40.9 56.3 56.9 354-370 bp 1.0 44.2
ndhE-ndhG_p3 AGTTCAAGACACATAAGAGCTCT TCGCTTTAATAGGTGCTATTGC 39.1 40.9 57.3 56.9 361-377 bp 1.0 44.2
ndhE-ndhG_p4 AGACACATAAGAGCTCTAACCA TCTTCCATTCGAACTCATCTCA 40.9 40.9 56.3 56.9 390-406 bp 1.0 43.9
ndhE-ndhG_p5 AGTTCAAGACACATAAGAGCTCT TCTTCCATTCGAACTCATCTCA 39.1 40.9 57.3 56.9 396-412 bp 1.0 43.8