Hotspot

ndhD-psaC

SSC · 118284-118427 · 144 bp

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Pi 0.0564
MarkerSeek score 74.40
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
144
variable_sites
12
pi
0.0564
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.90
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 TTTGCCCACTGTACATTGCT GATACCCTGGGACGGATGTA 45.0 55.0 58.0 58.0 669-684 bp 1.0 83.7
ndhD-psaC_p2 TTTGCCCACTGTACATTGCT AAATAGCTTCCGCTCCAAGG 45.0 50.0 58.0 58.0 640-655 bp 1.0 83.5
ndhD-psaC_p3 TTTGCCCACTGTACATTGCT AAATGATACCCTGGGACGGA 45.0 50.0 58.0 58.1 673-688 bp 1.0 82.8
ndhD-psaC_p4 TTTGCCCACTGTACATTGCT AATGATACCCTGGGACGGAT 45.0 50.0 58.0 57.9 672-687 bp 1.0 82.8
ndhD-psaC_p5 ATAATGCGCTTCTCCATGGG GATACCCTGGGACGGATGTA 50.0 55.0 58.1 58.0 1022-1037 bp 1.0 81.6