Hotspot

ccsA-ndhD

SSC · 116519-116780 · 262 bp

Back to Premna

Pi 0.037
MarkerSeek score 63.10
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
262
variable_sites
15
pi
0.037
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TTGGAGTTGGGACCCAAAAG TTTTTGATTCTGGACCGCGA 50.0 45.0 57.9 58.1 609-619 bp 1.0 82.2
ccsA-ndhD_p2 GAAGCGTGGGGATCCTATTG TTTTTGATTCTGGACCGCGA 55.0 45.0 58.1 58.1 626-636 bp 1.0 81.8
ccsA-ndhD_p3 TTGGAGTTGGGACCCAAAAG GCATTACCCGGTATGAGTGG 50.0 55.0 57.9 58.1 811-821 bp 1.0 81.2
ccsA-ndhD_p4 GAAGCGTGGGGATCCTATTG GCATTACCCGGTATGAGTGG 55.0 55.0 58.1 58.1 828-838 bp 1.0 80.9
ccsA-ndhD_p5 TTGGAGTTGGGACCCAAAAG CACGGATATGGGACTCAACG 50.0 55.0 57.9 58.1 1001-1011 bp 1.0 80.6