Hotspot

rpl32-trnL-UAG

SSC · 114916-115363 · 448 bp

Back to Premna

Pi 0.0324
MarkerSeek score 69.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
448
variable_sites
27
pi
0.0324
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAAACAAATAAACT TCCTAAGAGCAGCGTGTCTA 33.3 50.0 54.9 57.9 524-581 bp 1.0 51.4
rpl32-trnL-UAG_p2 TGTGCGACAAACAAATAAACT AGCAGCGTGTCTACCAATTT 33.3 45.0 54.9 57.8 517-574 bp 1.0 51.0
rpl32-trnL-UAG_p3 TGTGCGACAAACAAATAAACT CTAAGAGCAGCGTGTCTACC 33.3 55.0 54.9 57.8 522-579 bp 1.0 50.9
rpl32-trnL-UAG_p4 TGTGCGACAAACAAATAAACT CCTAAGAGCAGCGTGTCTAC 33.3 55.0 54.9 57.8 523-580 bp 1.0 50.8
rpl32-trnL-UAG_p5 TGTGCGACAAACAAATAAACT GAGCAGCGTGTCTACCAATT 33.3 50.0 54.9 58.3 518-575 bp 1.0 50.3