Hotspot

trnW-CCA-trnP-UGG

LSC · 68310-68481 · 172 bp

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Pi 0.0153
MarkerSeek score 63.10
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
172
variable_sites
5
pi
0.0153
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GTTTTGGAGACCCACGTTCT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 232-241 bp 1.0 83.6
trnW-CCA-trnP-UGG_p2 GTTTTGGAGACCCACGTTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 243-252 bp 1.0 83.5
trnW-CCA-trnP-UGG_p3 TTTTGGAGACCCACGTTCTG GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 242-251 bp 1.0 83.5
trnW-CCA-trnP-UGG_p4 TTTTGGAGACCCACGTTCTG TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 243-252 bp 1.0 83.5
trnW-CCA-trnP-UGG_p5 GTTTTGGAGACCCACGTTCT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 244-253 bp 1.0 83.4