Hotspot

ndhC-trnV-UAC

LSC · 52129-53209 · 1081 bp

Back to Premna

Pi 0.0165
MarkerSeek score 58.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1081
variable_sites
26
pi
0.0165
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.80
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATAGGGATAAGACTTGA AGAAGGTCTACGGTTCGAGT 37.5 50.0 57.9 57.8 1022-1186 bp 1.0 51.8
ndhC-trnV-UAC_p2 TGCCAAAATAGGGATAAGACTTGA GAAGGTCTACGGTTCGAGTC 37.5 55.0 57.9 57.5 1021-1185 bp 1.0 49.0
ndhC-trnV-UAC_p3 TGCCAAAATAGGGATAAGACTTG AGAAGGTCTACGGTTCGAGT 39.1 50.0 56.6 57.8 1022-1186 bp 1.0 48.9
ndhC-trnV-UAC_p4 TGCCAAAATAGGGATAAGACTTGA GTTCGAGTCCGTATAGCCCT 37.5 55.0 57.9 58.7 1010-1174 bp 1.0 47.9
ndhC-trnV-UAC_p5 GCCAAAATAGGGATAAGACTTGA AGAAGGTCTACGGTTCGAGT 39.1 50.0 56.3 57.8 1021-1185 bp 1.0 46.9