Hotspot

ycf3-trnS-GGA

LSC · 45829-46680 · 852 bp

Back to Premna

Pi 0.00984
MarkerSeek score 61.60
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
852
variable_sites
15
pi
0.00984
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGCCTCCTTTTCTCCTGAAG CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1024-1046 bp 1.0 75.4
ycf3-trnS-GGA_p2 GCCTCCTTTTCTCCTGAAGT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1023-1045 bp 1.0 75.4
ycf3-trnS-GGA_p3 TGCCTCCTTTTCTCCTGAAG ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.1 58.2 1045-1067 bp 1.0 74.9
ycf3-trnS-GGA_p4 GCCTCCTTTTCTCCTGAAGT ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.1 58.2 1044-1066 bp 1.0 74.9
ycf3-trnS-GGA_p5 TGCCTCCTTTTCTCCTGAAG TTCCAATGCTACGCCTTGAA 50.0 45.0 57.1 57.8 995-1017 bp 1.0 74.7