Hotspot

psaA-ycf3

LSC · 43051-43826 · 776 bp

Back to Premna

Pi 0.0148
MarkerSeek score 70.20
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
776
variable_sites
19
pi
0.0148
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 TGAGAAATGACCCGGTTTGG GGAAACAGGCTATAGCGCTT 50.0 50.0 58.1 58.0 934-968 bp 1.0 84.5
psaA-ycf3_p2 GCCCATTCCTCGAAAGAAGT GGAAACAGGCTATAGCGCTT 50.0 50.0 57.9 58.0 915-949 bp 1.0 84.3
psaA-ycf3_p3 TGAGAAATGACCCGGTTTGG TTGAAGATCACGAGGCGTTT 50.0 45.0 58.1 57.8 881-915 bp 1.0 83.6
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT TTGAAGATCACGAGGCGTTT 50.0 45.0 57.9 57.8 862-896 bp 1.0 83.5
psaA-ycf3_p5 TGAGAAATGACCCGGTTTGG TTGGTTGAAGATCACGAGGC 50.0 50.0 58.1 58.2 885-919 bp 1.0 83.4