Hotspot

trnE-UUC-trnT-GGU

LSC · 32061-32625 · 565 bp

Back to Premna

Pi 0.0182
MarkerSeek score 68.10
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
565
variable_sites
19
pi
0.0182
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 640-715 bp 1.0 84.9
trnE-UUC-trnT-GGU_p2 TTTCGTAGTACCCTACCCCC TACCGCTGAGTTAAAAGGGC 55.0 50.0 57.8 57.9 605-680 bp 1.0 84.2
trnE-UUC-trnT-GGU_p3 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 602-677 bp 1.0 84.2
trnE-UUC-trnT-GGU_p4 TTTCGTAGTACCCTACCCCC CCGATGACTTACGCCTTACC 55.0 55.0 57.8 58.2 637-712 bp 1.0 83.7
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG TACCGCTGAGTTAAAAGGGC 55.0 50.0 58.3 57.9 567-642 bp 1.0 83.5