Hotspot

petN-psbM

LSC · 30024-31020 · 997 bp

Back to Premna

Pi 0.0137
MarkerSeek score 61.00
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
997
variable_sites
24
pi
0.0137
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACTCGTAGTGTGGGGAAGAA AGTTCCTACCGCTTTTCTACT 50.0 42.9 58.0 56.4 1071-1086 bp 1.0 63.3
petN-psbM_p2 TCGTAGTGTGGGGAAGAAGT AGTTCCTACCGCTTTTCTACT 50.0 42.9 58.0 56.4 1069-1084 bp 1.0 63.3
petN-psbM_p3 ACTCGTAGTGTGGGGAAGAA TCATTCTAGTTCCTACCGCT 50.0 45.0 58.0 55.4 1078-1093 bp 1.0 63.1
petN-psbM_p4 TCGTAGTGTGGGGAAGAAGT TCATTCTAGTTCCTACCGCT 50.0 45.0 58.0 55.4 1076-1091 bp 1.0 63.1
petN-psbM_p5 CGTAGTGTGGGGAAGAAGTG AGTTCCTACCGCTTTTCTACT 55.0 42.9 57.9 56.4 1068-1083 bp 1.0 62.7