Hotspot

rps16-trnQ-UUG

LSC · 6398-7633 · 1236 bp

Back to Premna

Pi 0.0174
MarkerSeek score 67.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1236
variable_sites
36
pi
0.0174
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAAATTGAAATGGGGCGTGG 45.0 50.0 58.1 58.0 1323-1367 bp 1.0 84.4
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1265-1309 bp 1.0 84.1
rps16-trnQ-UUG_p3 GGATCGTGTCCTTCAAGTCG GAAATTGAAATGGGGCGTGG 55.0 50.0 58.4 58.0 1337-1381 bp 1.0 82.5
rps16-trnQ-UUG_p4 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1279-1323 bp 1.0 82.2
rps16-trnQ-UUG_p5 CGTTGCTTTCTACCACATCG GAAATTGAAATGGGGCGTGG 50.0 50.0 57.2 58.0 1315-1359 bp 1.0 79.3