Hotspot

ORF238

LSC · 32880-33596 · 717 bp

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Pi 0.247
MarkerSeek score 80.00
Primer available yes
Label ORF238

Feature Metadata

label_name
ORF238
feature_type
gene
region
LSC
length_bp
717
variable_sites
175
pi
0.247
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ORF238_p1 AGTCCCGCGGATACATAGAA GAAGCCTGTTGAGGAACCAA 50.0 50.0 58.0 58.0 2202-2447 bp 1.0 91.8
ORF238_p2 AGTCCCGCGGATACATAGAA GGTGCAGAGTCAAAAAGGGA 50.0 50.0 58.0 58.0 2230-2475 bp 1.0 91.8
ORF238_p3 ATTGGATCCCCACCTACACA GAAGCCTGTTGAGGAACCAA 50.0 50.0 58.0 58.0 2658-2903 bp 1.0 91.7
ORF238_p4 ATTGGATCCCCACCTACACA GGTGCAGAGTCAAAAAGGGA 50.0 50.0 58.0 58.0 2686-2931 bp 1.0 91.7
ORF238_p5 AGAACCCCACCACATACTCA GAAGCCTGTTGAGGAACCAA 50.0 50.0 58.0 58.0 2022 bp 0.5 59.5