Hotspot

ORF224

LSC · 56545-57219 · 675 bp

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Pi 0.0711
MarkerSeek score 75.20
Primer available yes
Label ORF224

Feature Metadata

label_name
ORF224
feature_type
gene
region
LSC
length_bp
675
variable_sites
48
pi
0.0711
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ORF224_p1 CTAGAAAGTTTCTCCGGCCC AGCGTTCTGAAAATCCGTGT 55.0 45.0 58.0 58.1 1141-1296 bp 1.0 84.7
ORF224_p2 GGGGCTAAAACTCCCGAAAT AGCGTTCTGAAAATCCGTGT 50.0 45.0 57.9 58.1 1111-1266 bp 1.0 83.9
ORF224_p3 TCGGAGCTAAGACCATTCCA AGCGTTCTGAAAATCCGTGT 50.0 45.0 58.1 58.1 1382-1537 bp 1.0 82.8
ORF224_p4 CTAAAAACCCGCAGCCAAAG AGCGTTCTGAAAATCCGTGT 50.0 45.0 57.9 58.1 743 bp 0.5 58.5
ORF224_p5 CTTCTTTTCATCGCAGTCGC AGCGTTCTGAAAATCCGTGT 50.0 45.0 57.8 58.1 945 bp 0.5 57.8