Hotspot

psaC-ndhD

SSC · 120423-120534 · 112 bp

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Pi 0.0804
MarkerSeek score 73.20
Primer available yes
Label psaC-ndhD

Feature Metadata

label_name
psaC-ndhD
feature_type
igs
region
SSC
length_bp
112
variable_sites
9
pi
0.0804
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhD_p1 GCAGCATGGGTCTAGCTTAT AAGAACCCGCAGTTATTGGT 50.0 45.0 57.4 57.4 193 bp 1.0 74.6
psaC-ndhD_p2 ATGAAACAACTCGCAGCATG AAGAACCCGCAGTTATTGGT 45.0 45.0 57.4 57.4 205 bp 1.0 73.9
psaC-ndhD_p3 CATGAAACAACTCGCAGCAT AAGAACCCGCAGTTATTGGT 45.0 45.0 57.4 57.4 206 bp 1.0 73.9
psaC-ndhD_p4 ATGGCATGAAACAACTCGCA AAGAACCCGCAGTTATTGGT 45.0 45.0 58.8 57.4 210 bp 1.0 72.8
psaC-ndhD_p5 GCAGCATGGGTCTAGCTTAT AAAGAACCCGCAGTTATTGGT 50.0 42.9 57.4 58.1 194 bp 1.0 70.2