Hotspot

rpoB-ORF238

LSC · 32502-32879 · 378 bp

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Pi 0.0989
MarkerSeek score 75.00
Primer available yes
Label rpoB-ORF238

Feature Metadata

label_name
rpoB-ORF238
feature_type
igs
region
LSC
length_bp
378
variable_sites
37
pi
0.0989
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-ORF238_p1 AGTCCCGCGGATACATAGAA CGCGGAAATGTTTTCAGACC 50.0 50.0 58.0 58.0 855 bp 0.5 59.6
rpoB-ORF238_p2 ATTGGATCCCCACCTACACA CGCGGAAATGTTTTCAGACC 50.0 50.0 58.0 58.0 1311 bp 0.5 59.5
rpoB-ORF238_p3 AGTCCCGCGGATACATAGAA ACACCAGAACCTGACAAACC 50.0 50.0 58.0 57.9 1169 bp 0.5 59.5
rpoB-ORF238_p4 AGTCCCGCGGATACATAGAA CTACAGGAGGGACACCAGAA 50.0 55.0 58.0 58.1 1180 bp 0.5 59.4
rpoB-ORF238_p5 ATTGGATCCCCACCTACACA ACACCAGAACCTGACAAACC 50.0 50.0 58.0 57.9 1625 bp 0.5 59.4