primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-rbcL_p1	psbA-rbcL	1	TACAGAAGCGACCCCATAGG	ATTTGAGTTTGCCGCAATGG	20	20	55.0	45.0	58.6	57.9	0.0	6.6	0.673162	1497	2538	1128-1129	1.000000	77.9
psbA-rbcL_p2	psbA-rbcL	2	TACAGAAGCGACCCCATAGG	TGAGTTTGCCGCAATGGATA	20	20	55.0	45.0	58.6	57.9	0.0	6.6	0.711287	1497	2538	1125-1126	1.000000	77.6
psbA-rbcL_p3	psbA-rbcL	3	TACAGAAGCGACCCCATAGG	GCTGCTTGTGAGGTATGGAA	20	20	55.0	50.0	58.6	57.9	0.0	0.0	0.703856	1497	2538	1157-1158	1.000000	77.5
psbA-rbcL_p4	psbA-rbcL	4	TACAGAAGCGACCCCATAGG	AGTTTGCCGCAATGGATACT	20	20	55.0	45.0	58.6	57.9	0.0	6.6	0.718766	1497	2538	1123-1124	1.000000	77.5
psbA-rbcL_p5	psbA-rbcL	5	ATCCAGTTACAGAAGCGACC	ATTTGAGTTTGCCGCAATGG	20	20	50.0	45.0	57.3	57.9	0.0	6.6	0.757962	1497	2538	1135-1136	1.000000	77.2
ORF192_p1	ORF192	1	AGCGAACATCAATTGCAACG	GTCCCGGTAATCGAAGGTTC	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.100332	11751	12329	1064-1134	1.000000	89.3
ORF192_p2	ORF192	2	GCGAACATCAATTGCAACGA	GTCCCGGTAATCGAAGGTTC	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.105682	11751	12329	1063-1133	1.000000	89.3
ORF192_p3	ORF192	3	AGCGAACATCAATTGCAACG	AAGGATTTGGGCAGGTCTTG	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.103370	11751	12329	1256-1266	1.000000	88.7
ORF192_p4	ORF192	4	AGCGAACATCAATTGCAACG	GCAGGTCTTGACACCAAAGA	20	20	45.0	50.0	58.0	58.0	0.0	3.1	0.064920	11751	12329	1246-1246	0.500000	59.5
ORF192_p5	ORF192	5	GCGAACATCAATTGCAACGA	GCAGGTCTTGACACCAAAGA	20	20	45.0	50.0	58.0	58.0	0.0	3.1	0.070270	11751	12329	1245-1245	0.500000	59.4
trnS (GCU)-trnG (UCC)_p1	trnS (GCU)-trnG (UCC)	1	ACGGAAAGAGAGGGATTCGA	AGGAGGTTCATAGAACGAATCAC	20	23	50.0	43.5	58.2	58.0	0.0	4.0	3.163677	14018	14756	860-876	1.000000	59.2
trnS (GCU)-trnG (UCC)_p2	trnS (GCU)-trnG (UCC)	2	ACGAAAACGGAAAGAGAGGG	AGGAGGTTCATAGAACGAATCAC	20	23	50.0	43.5	57.8	58.0	0.0	4.0	3.177647	14018	14756	866-882	1.000000	59.1
trnS (GCU)-trnG (UCC)_p3	trnS (GCU)-trnG (UCC)	3	ACGGAAAGAGAGGGATTCGA	GGAGGTTCATAGAACGAATCACA	20	23	50.0	43.5	58.2	58.2	0.0	4.0	3.395937	14018	14756	859-875	1.000000	57.4
trnS (GCU)-trnG (UCC)_p4	trnS (GCU)-trnG (UCC)	4	ACGAAAACGGAAAGAGAGGG	GGAGGTTCATAGAACGAATCACA	20	23	50.0	43.5	57.8	58.2	0.0	4.0	3.409907	14018	14756	865-881	1.000000	57.2
trnS (GCU)-trnG (UCC)_p5	trnS (GCU)-trnG (UCC)	5	AGTCATCGACGAAAACGGAA	AGGAGGTTCATAGAACGAATCAC	20	23	45.0	43.5	57.6	58.0	0.0	4.0	3.439560	14018	14756	874-890	1.000000	56.9
rpoB-ORF238_p1	rpoB-ORF238	1	AGTCCCGCGGATACATAGAA	CGCGGAAATGTTTTCAGACC	20	20	50.0	50.0	58.0	58.0	30.7	0.0	0.045132	32502	32879	855-855	0.500000	59.6
rpoB-ORF238_p2	rpoB-ORF238	2	ATTGGATCCCCACCTACACA	CGCGGAAATGTTTTCAGACC	20	20	50.0	50.0	58.0	58.0	9.9	0.0	0.056444	32502	32879	1311-1311	0.500000	59.5
rpoB-ORF238_p3	rpoB-ORF238	3	AGTCCCGCGGATACATAGAA	ACACCAGAACCTGACAAACC	20	20	50.0	50.0	58.0	57.9	30.7	0.0	0.061661	32502	32879	1169-1169	0.500000	59.5
rpoB-ORF238_p4	rpoB-ORF238	4	AGTCCCGCGGATACATAGAA	CTACAGGAGGGACACCAGAA	20	20	50.0	55.0	58.0	58.1	30.7	0.0	0.070294	32502	32879	1180-1180	0.500000	59.4
rpoB-ORF238_p5	rpoB-ORF238	5	ATTGGATCCCCACCTACACA	ACACCAGAACCTGACAAACC	20	20	50.0	50.0	58.0	57.9	9.9	0.0	0.072973	32502	32879	1625-1625	0.500000	59.4
ORF238_p1	ORF238	1	AGTCCCGCGGATACATAGAA	GAAGCCTGTTGAGGAACCAA	20	20	50.0	50.0	58.0	58.0	30.7	0.0	0.027622	32880	33596	2202-2447	1.000000	91.8
ORF238_p2	ORF238	2	AGTCCCGCGGATACATAGAA	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.0	58.0	30.7	0.0	0.027622	32880	33596	2230-2475	1.000000	91.8
ORF238_p3	ORF238	3	ATTGGATCCCCACCTACACA	GAAGCCTGTTGAGGAACCAA	20	20	50.0	50.0	58.0	58.0	9.9	0.0	0.038934	32880	33596	2658-2903	1.000000	91.7
ORF238_p4	ORF238	4	ATTGGATCCCCACCTACACA	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.0	58.0	9.9	0.0	0.038934	32880	33596	2686-2931	1.000000	91.7
ORF238_p5	ORF238	5	AGAACCCCACCACATACTCA	GAAGCCTGTTGAGGAACCAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.057606	32880	33596	2022-2022	0.500000	59.5
ORF238-trnC (GCA)_p1	ORF238-trnC (GCA)	1	GGTCTGAAAACATTTCCGCG	GAAGCCTGTTGAGGAACCAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054997	33597	34450	1612-1612	0.500000	59.6
ORF238-trnC (GCA)_p2	ORF238-trnC (GCA)	2	GGTCTGAAAACATTTCCGCG	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054997	33597	34450	1640-1640	0.500000	59.6
ORF238-trnC (GCA)_p3	ORF238-trnC (GCA)	3	GGTTTGTCAGGTTCTGGTGT	GAAGCCTGTTGAGGAACCAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.071526	33597	34450	1298-1298	0.500000	59.4
ORF238-trnC (GCA)_p4	ORF238-trnC (GCA)	4	GGTTTGTCAGGTTCTGGTGT	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.071526	33597	34450	1326-1326	0.500000	59.4
ORF238-trnC (GCA)_p5	ORF238-trnC (GCA)	5	TTCTGGTGTCCCTCCTGTAG	GAAGCCTGTTGAGGAACCAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.080159	33597	34450	1287-1287	0.500000	59.4
trnC (GCA)-petN_p1	trnC (GCA)-petN	1	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	34522	34788	341-342	1.000000	80.4
trnC (GCA)-petN_p2	trnC (GCA)-petN	2	GGGGGACTGCAAATCCTTTT	CCCAAGCGAGACTTACTAGC	20	20	50.0	55.0	58.4	57.8	14.7	0.0	0.566843	34522	34788	339-340	1.000000	79.9
trnC (GCA)-petN_p3	trnC (GCA)-petN	3	GGGGGACTGCAAATCCTTTT	GCCCAAGCGAGACTTACTAG	20	20	50.0	55.0	58.4	57.8	14.7	0.0	0.566843	34522	34788	340-341	1.000000	79.9
trnC (GCA)-petN_p4	trnC (GCA)-petN	4	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.5	58.2	0.0	0.0	0.627590	34522	34788	316-317	1.000000	79.8
trnC (GCA)-petN_p5	trnC (GCA)-petN	5	AGTTCAAATCTGGGTGTCGC	CCCAAGCGAGACTTACTAGC	20	20	50.0	55.0	58.5	57.8	0.0	0.0	0.687595	34522	34788	314-315	1.000000	79.3
trnT (UGU)-trnL (UAA)_p1	trnT (UGU)-trnL (UAA)	1	GCGATGCTCTACCTTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	52277	52955	725-729	1.000000	83.2
trnT (UGU)-trnL (UAA)_p2	trnT (UGU)-trnL (UAA)	2	GCGATGCTCTACCTTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	52277	52955	733-737	1.000000	82.6
trnT (UGU)-trnL (UAA)_p3	trnT (UGU)-trnL (UAA)	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	52277	52955	757-761	1.000000	82.1
trnT (UGU)-trnL (UAA)_p4	trnT (UGU)-trnL (UAA)	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	52277	52955	765-769	1.000000	81.6
trnT (UGU)-trnL (UAA)_p5	trnT (UGU)-trnL (UAA)	5	GCGATGCTCTACCTTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	52277	52955	739-743	1.000000	81.2
ndhC-ORF224_p1	ndhC-ORF224	1	CTAGAAAGTTTCTCCGGCCC	CGGGCAAAGAAGAAATGCAG	20	20	55.0	50.0	58.0	58.0	21.2	0.0	0.038891	56238	56544	515-670	1.000000	84.7
ndhC-ORF224_p2	ndhC-ORF224	2	CTAGAAAGTTTCTCCGGCCC	GTCGGGCAAAGAAGAAATGC	20	20	55.0	50.0	58.0	58.0	21.2	0.0	0.042976	56238	56544	517-672	1.000000	84.6
ndhC-ORF224_p3	ndhC-ORF224	3	GTGAGCCGATTAGTGAAGCA	CGGGCAAAGAAGAAATGCAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023447	56238	56544	962-1117	1.000000	82.9
ndhC-ORF224_p4	ndhC-ORF224	4	GTGAGCCGATTAGTGAAGCA	GTCGGGCAAAGAAGAAATGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.027533	56238	56544	964-1119	1.000000	82.9
ndhC-ORF224_p5	ndhC-ORF224	5	GTGAGCCGATTAGTGAAGCA	ACGTCTGAAAAACCCCTCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.046973	56238	56544	1331-1331	0.500000	59.6
ORF224_p1	ORF224	1	CTAGAAAGTTTCTCCGGCCC	AGCGTTCTGAAAATCCGTGT	20	20	55.0	45.0	58.0	58.1	21.2	0.0	0.146636	56545	57219	1141-1296	1.000000	84.7
ORF224_p2	ORF224	2	GGGGCTAAAACTCCCGAAAT	AGCGTTCTGAAAATCCGTGT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.260370	56545	57219	1111-1266	1.000000	83.9
ORF224_p3	ORF224	3	TCGGAGCTAAGACCATTCCA	AGCGTTCTGAAAATCCGTGT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.260406	56545	57219	1382-1537	1.000000	82.8
ORF224_p4	ORF224	4	CTAAAAACCCGCAGCCAAAG	AGCGTTCTGAAAATCCGTGT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.185620	56545	57219	743-743	0.500000	58.5
ORF224_p5	ORF224	5	CTTCTTTTCATCGCAGTCGC	AGCGTTCTGAAAATCCGTGT	20	20	50.0	45.0	57.8	58.1	0.0	0.0	0.271844	56545	57219	945-945	0.500000	57.8
rpoA-rps11_p1	rpoA-rps11	1	CACACTTCCACTGGAGTGTC	GCCCCATAATGGCTGTAGAC	20	20	55.0	55.0	58.1	58.1	21.3	0.0	0.231360	77212	77291	175-175	1.000000	80.0
rpoA-rps11_p2	rpoA-rps11	2	CACACTTCCACTGGAGTGTC	ACGGGATGTAACTCCTTTGC	20	20	55.0	50.0	58.1	57.9	21.3	0.0	0.238345	77212	77291	193-193	1.000000	79.8
rpoA-rps11_p3	rpoA-rps11	3	CACACTTCCACTGGAGTGTC	GGATGTAACTCCTTTGCCCC	20	20	55.0	55.0	58.1	58.2	21.3	0.0	0.364952	77212	77291	190-190	1.000000	78.8
rpoA-rps11_p4	rpoA-rps11	4	CACACTTCCACTGGAGTGTC	ACGCAGCATTACGAGCTATT	20	20	55.0	45.0	58.1	57.8	21.3	0.0	0.353988	77212	77291	242-242	1.000000	78.5
rpoA-rps11_p5	rpoA-rps11	5	CACACTTCCACTGGAGTGTC	GACGCAGCATTACGAGCTAT	20	20	55.0	50.0	58.1	58.2	21.3	0.0	0.356737	77212	77291	243-243	1.000000	78.5
trnI (CAU)-ycf2_p1	trnI (CAU)-ycf2	1	TGAACCTACGAATTCGCCAA	CAGAACCTTACGAAAGATTGCG	20	22	45.0	45.5	57.5	58.0	27.0	0.0	2.467992	84991	85112	265-266	1.000000	61.8
trnI (CAU)-ycf2_p2	trnI (CAU)-ycf2	2	TTGAACCTACGAATTCGCCA	CAGAACCTTACGAAAGATTGCG	20	22	45.0	45.5	57.5	58.0	27.0	0.0	2.467992	84991	85112	266-267	1.000000	61.8
trnI (CAU)-ycf2_p3	trnI (CAU)-ycf2	3	TGAACCTACGAATTCGCCAA	AGAACCTTACGAAAGATTGCGA	20	22	45.0	40.9	57.5	58.1	27.0	0.0	2.537383	84991	85112	264-265	1.000000	61.2
trnI (CAU)-ycf2_p4	trnI (CAU)-ycf2	4	TTGAACCTACGAATTCGCCA	AGAACCTTACGAAAGATTGCGA	20	22	45.0	40.9	57.5	58.1	27.0	0.0	2.537383	84991	85112	265-266	1.000000	61.2
trnI (CAU)-ycf2_p5	trnI (CAU)-ycf2	5	GCCAATTATGAGTTGGGTGC	CAGAACCTTACGAAAGATTGCG	20	22	50.0	45.5	57.4	58.0	7.0	0.0	2.593423	84991	85112	250-251	1.000000	60.8
psaC-ndhD_p1	psaC-ndhD	1	GCAGCATGGGTCTAGCTTAT	AAGAACCCGCAGTTATTGGT	20	20	50.0	45.0	57.4	57.4	0.0	0.0	1.137329	120423	120534	193-193	1.000000	74.6
psaC-ndhD_p2	psaC-ndhD	2	ATGAAACAACTCGCAGCATG	AAGAACCCGCAGTTATTGGT	20	20	45.0	45.0	57.4	57.4	0.0	0.0	1.203726	120423	120534	205-205	1.000000	73.9
psaC-ndhD_p3	psaC-ndhD	3	CATGAAACAACTCGCAGCAT	AAGAACCCGCAGTTATTGGT	20	20	45.0	45.0	57.4	57.4	0.0	0.0	1.203726	120423	120534	206-206	1.000000	73.9
psaC-ndhD_p4	psaC-ndhD	4	ATGGCATGAAACAACTCGCA	AAGAACCCGCAGTTATTGGT	20	20	45.0	45.0	58.8	57.4	0.0	0.0	1.332761	120423	120534	210-210	1.000000	72.8
psaC-ndhD_p5	psaC-ndhD	5	GCAGCATGGGTCTAGCTTAT	AAAGAACCCGCAGTTATTGGT	20	21	50.0	42.9	57.4	58.1	0.0	0.0	1.686467	120423	120534	194-194	1.000000	70.2
trnL (UAG)-rpl32_p1	trnL (UAG)-rpl32	1	AGCGTGTCTACCGATTTCAC	GCGGATTCGTTGGACTAGTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013801	123383	124075	1336-1336	0.500000	59.9
trnL (UAG)-rpl32_p2	trnL (UAG)-rpl32	2	CAGCGTGTCTACCGATTTCA	GCGGATTCGTTGGACTAGTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.015171	123383	124075	1337-1337	0.500000	59.9
trnL (UAG)-rpl32_p3	trnL (UAG)-rpl32	3	GGTGTTTTGAAGACGGCATG	GCGGATTCGTTGGACTAGTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.056448	123383	124075	1405-1405	0.500000	59.5
trnL (UAG)-rpl32_p4	trnL (UAG)-rpl32	4	AGCGTGTCTACCGATTTCAC	CTTTCGACCGGCAAGTCTAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.082377	123383	124075	776-776	0.500000	59.3
trnL (UAG)-rpl32_p5	trnL (UAG)-rpl32	5	AGCGTGTCTACCGATTTCAC	TCGACCGGCAAGTCTAAAAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.082377	123383	124075	773-773	0.500000	59.3
rpl32-ndhF_p1	rpl32-ndhF	1	GAGCCAATTTTCGCGTCAAA	TACATGAAGGGGGAATTGCG	20	20	45.0	50.0	58.0	57.9	5.8	0.0	0.096222	124241	125360	2073-2092	1.000000	82.4
rpl32-ndhF_p2	rpl32-ndhF	2	GAGCCAATTTTCGCGTCAAA	AACCAAAATCCCCAACACGA	20	20	45.0	45.0	58.0	57.9	5.8	0.0	0.119617	124241	125360	1894-1913	1.000000	82.3
rpl32-ndhF_p3	rpl32-ndhF	3	GAGCCAATTTTCGCGTCAAA	TGTCGCTGACGGAAAAAGAA	20	20	45.0	45.0	58.0	58.1	5.8	4.6	0.102211	124241	125360	1428-1428	0.500000	59.2
rpl32-ndhF_p4	rpl32-ndhF	4	GAGCCAATTTTCGCGTCAAA	TTGTCGCTGACGGAAAAAGA	20	20	45.0	45.0	58.0	58.1	5.8	4.6	0.102211	124241	125360	1429-1429	0.500000	59.2
rpl32-ndhF_p5	rpl32-ndhF	5	TTAGACTTGCCGGTCGAAAG	TACATGAAGGGGGAATTGCG	20	20	50.0	50.0	57.9	57.9	1.9	0.0	0.125881	124241	125360	1995-1995	0.500000	59.0