Hotspot

ycf1

SSC · 124823-130306 · 5484 bp

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Pi 0.0093

MarkerSeek score 44.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5484
variable_sites: 51
pi: 0.0093
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTTGGTTAGTTTCGCCGGA	CCCCCAAACCTTCCTTTTT	45.0	47.4	58.0	55.4	265 bp	1.0	52.7
ycf1_p2	TTGGTTAGTTTCGCCGGATT	CCCCCAAACCTTCCTTTTT	45.0	47.4	57.8	55.4	263 bp	1.0	51.5
ycf1_p3	TTTGGTTAGTTTCGCCGGAT	CCCCCAAACCTTCCTTTTT	45.0	47.4	57.8	55.4	264 bp	1.0	51.5
ycf1_p4	GGTTAGTTTCGCCGGATTCA	CCCCCAAACCTTCCTTTTT	50.0	47.4	58.3	55.4	261 bp	1.0	50.8
ycf1_p5	TGGTTAGTTTCGCCGGATTC	CCCCCAAACCTTCCTTTTT	50.0	47.4	58.3	55.4	262 bp	1.0	50.8