Hotspot

rps8

LSC · 80552-80956 · 405 bp

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Pi 0.00247

MarkerSeek score 42.30

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 1
pi: 0.00247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	TCCAGGTAAAGCCTCCTTGA	GCAAGATCAGAGGTGGTCAG	50.0	55.0	58.0	58.0	940 bp	1.0	80.4
rps8_p2	TCCAGGTAAAGCCTCCTTGA	AAACAATAACAGGTGCCGGT	50.0	45.0	58.0	58.0	1194-1196 bp	1.0	80.3
rps8_p3	TCCAGGTAAAGCCTCCTTGA	AACAATAACAGGTGCCGGTT	50.0	45.0	58.0	58.0	1193-1195 bp	1.0	80.3
rps8_p4	TCCAGGTAAAGCCTCCTTGA	ACAATAACAGGTGCCGGTTT	50.0	45.0	58.0	58.0	1192-1194 bp	1.0	80.3
rps8_p5	AAGCGAATCCGGAACATACC	ACAATAACAGGTGCCGGTTT	50.0	45.0	58.1	58.0	1266-1268 bp	1.0	80.3