Hotspot

petB

LSC · 75380-76777 · 1398 bp

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Pi 0.0143

MarkerSeek score 53.00

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1398
variable_sites: 20
pi: 0.0143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AACAGCTCCTTTGATGGGTG	ATGCGGCAGATGGATAAAGG	50.0	50.0	58.1	58.1	1884-1889 bp	1.0	79.6
petB_p2	AACAGCTCCTTTGATGGGTG	CGGCAGATGGATAAAGGCAT	50.0	50.0	58.1	58.1	1881-1886 bp	1.0	79.6
petB_p3	AACAGCTCCTTTGATGGGTG	TTCCAATGTAATGCGGCAGA	50.0	45.0	58.1	57.9	1894-1899 bp	1.0	79.4
petB_p4	TGTTGAGAAGGTTGCCCAAA	ATGCGGCAGATGGATAAAGG	45.0	50.0	57.8	58.1	2197-2202 bp	1.0	78.9
petB_p5	TGTTGAGAAGGTTGCCCAAA	CGGCAGATGGATAAAGGCAT	45.0	50.0	57.8	58.1	2194-2199 bp	1.0	78.9