Hotspot

petN-psbM

LSC · 28905-30020 · 1116 bp

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Pi 0.00811

MarkerSeek score 63.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1116
variable_sites: 9
pi: 0.00811
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1290-1306 bp	1.0	68.5
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1229-1245 bp	1.0	66.9
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCTTTTCTGCT	50.0	42.9	58.2	57.2	1269-1285 bp	1.0	65.5
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	1290-1306 bp	1.0	64.3
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCTTTTCTGCT	50.0	42.9	57.6	57.2	1208-1224 bp	1.0	63.9