Hotspot

atpF-atpH

LSC · 13167-13447 · 281 bp

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Pi 0.0214

MarkerSeek score 64.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 281
variable_sites: 6
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	397-402 bp	1.0	78.3
atpF-atpH_p2	CCAGTGGCCTAAAGAAACGA	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.8	58.2	370-375 bp	1.0	77.9
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	399-404 bp	1.0	76.9
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	TGAGGCGGAGGGAAAAATAC	50.0	50.0	57.9	57.6	468-473 bp	1.0	76.8
atpF-atpH_p5	CCAGTGGCCTAAAGAAACGA	TGAGGCGGAGGGAAAAATAC	50.0	50.0	57.8	57.6	441-446 bp	1.0	76.3