Hotspot

trnK-UUU-rps16

LSC · 4367-5095 · 729 bp

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Pi 0.0138
MarkerSeek score 53.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
729
variable_sites
10
pi
0.0138
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG CGATTCAAAGTGTATCGCGC 55.0 50.0 58.0 58.0 1590-1591 bp 1.0 80.9
trnK-UUU-rps16_p2 CCCTTTCAGGATCAGTCGTG GATTCAAAGTGTATCGCGCG 55.0 50.0 58.0 58.0 1589-1590 bp 1.0 80.9
trnK-UUU-rps16_p3 CCTTTCAGGATCAGTCGTGG CGATTCAAAGTGTATCGCGC 55.0 50.0 58.0 58.0 1589-1590 bp 1.0 80.9
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG GATTCAAAGTGTATCGCGCG 55.0 50.0 58.0 58.0 1588-1589 bp 1.0 80.9
trnK-UUU-rps16_p5 CGAATCCTCGCTTCATCCAA GATTCAAAGTGTATCGCGCG 50.0 50.0 58.1 58.0 1541-1542 bp 1.0 80.6