Hotspot

rpl32-trnL-UAG

SSC · 113237-114070 · 834 bp

Back to Pouzolzia

Pi 0.0777
MarkerSeek score 85.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
834
variable_sites
91
pi
0.0777
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAGCATTGAAAGCCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 940-976 bp 1.0 90.4
rpl32-trnL-UAG_p2 GGCAGCATTGAAAGCCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 941-977 bp 1.0 90.4
rpl32-trnL-UAG_p3 AGCCCGGCCAATTAAATGAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 1520 bp 0.333333 52.1
rpl32-trnL-UAG_p4 AGCCCGGCCAATTAAATGAA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.1 58.0 1521 bp 0.333333 52.1
rpl32-trnL-UAG_p5 AGCCCGGCCAATTAAATGAA GCTGCATAGCCTAACACCAT 45.0 50.0 58.1 58.0 2074 bp 0.333333 52.0