Hotspot

rpl36-rps8

IRb · 79331-80156 · 826 bp

Back to Pouzolzia

Pi 0.265

MarkerSeek score 72.90

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: IRb
length_bp: 826
variable_sites: 152
pi: 0.265
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.48
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	AATTTTACGGACGGAGGCTC	TAGATTGGTTGGGTCGCATG	50.0	50.0	58.0	58.0	1376 bp	0.333333	52.9
rpl36-rps8_p2	AATTTTACGGACGGAGGCTC	GTAGATTGGTTGGGTCGCAT	50.0	50.0	58.0	58.0	1377 bp	0.333333	52.9
rpl36-rps8_p3	CCCTTAATGCCGCATCTCTT	TAGATTGGTTGGGTCGCATG	50.0	50.0	58.0	58.0	1900 bp	0.333333	52.8
rpl36-rps8_p4	CCCTTAATGCCGCATCTCTT	GTAGATTGGTTGGGTCGCAT	50.0	50.0	58.0	58.0	1901 bp	0.333333	52.8
rpl36-rps8_p5	AGGACCATGAAATTACCCGC	TAGATTGGTTGGGTCGCATG	50.0	50.0	57.9	58.0	1741 bp	0.333333	52.6