Hotspot

trnF-GAA-ndhJ

LSC · 48283-48825 · 543 bp

Back to Pouzolzia

Pi 0.0781

MarkerSeek score 87.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 543
variable_sites: 60
pi: 0.0781
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	636-646 bp	1.0	80.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	635-645 bp	1.0	80.1
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	TGGATAGGATGGCCTTTACG	55.0	50.0	58.0	56.8	672-682 bp	1.0	80.0
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	GGATAGGATGGCCTTTACGT	55.0	50.0	58.0	56.8	671-681 bp	1.0	80.0
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGATAGGATGGCC	55.0	47.6	58.0	57.7	679-689 bp	1.0	79.3