Hotspot

trnL-UAA-trnF-GAA

LSC · 47824-48209 · 386 bp

Back to Pouzolzia

Pi 0.0557

MarkerSeek score 81.30

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 32
pi: 0.0557
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	ATCGTGAGGGTTCAAATCCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.6	58.0	428-439 bp	1.0	84.7
trnL-UAA-trnF-GAA_p2	ATCGTGAGGGTTCAAATCCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.6	58.1	464-475 bp	1.0	83.7
trnL-UAA-trnF-GAA_p3	ATCGTGAGGGTTCAAATCCC	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.6	57.9	440-451 bp	1.0	83.4
trnL-UAA-trnF-GAA_p4	ATCGTGAGGGTTCAAATCCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.6	58.1	479-490 bp	1.0	83.2
trnL-UAA-trnF-GAA_p5	ATCGTGAGGGTTCAAATCCC	CATGTGCCAGGAACCAGATT	50.0	50.0	57.6	58.2	482-493 bp	1.0	82.6