Hotspot

trnC-GCA-petN

LSC · 27381-28263 · 883 bp

Back to Pouzolzia

Pi 0.0837
MarkerSeek score 84.10
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
883
variable_sites
91
pi
0.0837
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCGAGACTTATT 50.0 50.0 58.1 58.0 651-950 bp 1.0 91.1
trnC-GCA-petN_p2 GGGTGTCGCCTGATTAACAA CAGCCCAAGCGAGACTTATT 50.0 50.0 58.2 58.0 633-932 bp 1.0 90.3
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTT CAGCCCAAGCGAGACTTATT 50.0 50.0 58.4 58.0 669-968 bp 1.0 88.9
trnC-GCA-petN_p4 CCAGTTCAAATCCGGGTGTC CAGCCCAAGCGAGACTTATT 55.0 50.0 58.8 58.0 646-945 bp 1.0 85.1
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG ATTAAAGCAGCCCAAGCGAG 50.0 50.0 58.1 58.9 658-957 bp 1.0 84.1