Hotspot

rps16-trnQ-UUG

LSC · 6351-6987 · 637 bp

Back to Pouzolzia

Pi 0.0634

MarkerSeek score 85.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 637
variable_sites: 56
pi: 0.0634
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	692-719 bp	1.0	88.6
rps16-trnQ-UUG_p2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	707-734 bp	1.0	86.6
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	706-733 bp	1.0	86.6
rps16-trnQ-UUG_p4	TGGGTGTAAGAATCCACAGC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	726-753 bp	1.0	85.5
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.1	58.5	713-740 bp	1.0	84.8