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trnK-UUU-rps16

LSC · 4317-5181 · 865 bp

Back to Pouzolzia

Pi 0.0693

MarkerSeek score 83.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 865
variable_sites: 85
pi: 0.0693
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GACGACATGCTGCTTTTTCC	TTTTCTTGAGCCGTACGAGG	50.0	50.0	58.0	57.9	1268-1297 bp	1.0	89.7
trnK-UUU-rps16_p2	GACGACATGCTGCTTTTTCC	GCCGTACGAGGAGAAAACTT	50.0	50.0	58.0	57.9	1259-1288 bp	1.0	89.7
trnK-UUU-rps16_p3	GACGACATGCTGCTTTTTCC	TTTGGTTGGGCAACGAAAAC	50.0	45.0	58.0	58.0	1429 bp	0.333333	53.0
trnK-UUU-rps16_p4	CTGTCCTCCCCCTTTTCAAC	TTTGGTTGGGCAACGAAAAC	55.0	45.0	58.1	58.0	1942 bp	0.333333	52.5
trnK-UUU-rps16_p5	ATTCAGACGACATGCTGCTT	TTTGGTTGGGCAACGAAAAC	45.0	45.0	57.9	58.0	1434 bp	0.333333	52.4