Hotspot

matK-trnK-UUU

LSC · 3484-4279 · 796 bp

Back to Pouzolzia

Pi 0.0687
MarkerSeek score 85.30
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
796
variable_sites
76
pi
0.0687
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 AATATCCTTGAAACTCTTCCA AACGGTAGAGTACTCGGCTT 33.3 50.0 52.1 58.2 790-842 bp 1.0 51.4
matK-trnK-UUU_p2 AATATCCTTGAAACTCTTCCA ACGGTAGAGTACTCGGCTTT 33.3 50.0 52.1 58.2 789-841 bp 1.0 51.4
matK-trnK-UUU_p3 AAATATCCTTGAAACTCTTCCA AACGGTAGAGTACTCGGCTT 31.8 50.0 53.0 58.2 791-843 bp 1.0 51.4
matK-trnK-UUU_p4 AAATATCCTTGAAACTCTTCCA ACGGTAGAGTACTCGGCTTT 31.8 50.0 53.0 58.2 790-842 bp 1.0 51.4
matK-trnK-UUU_p5 AATATCCTTGAAACTCTTCCA GGGTTGCTAACTCAACGGTA 33.3 50.0 52.1 57.5 803-855 bp 1.0 51.3