Hotspot

petB

LSC · 78672-80103 · 1432 bp

Back to Pouteria

Pi 0.00607

MarkerSeek score 62.90

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1432
variable_sites: 13
pi: 0.00607
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AAGAACCACAACGTCCTAGC	GTCTTGTTACTCCCCTAAGTGA	50.0	45.5	57.8	56.8	1599-1608 bp	1.0	54.6
petB_p2	GAACCACAACGTCCTAGCTT	GTCTTGTTACTCCCCTAAGTGA	50.0	45.5	57.8	56.8	1597-1606 bp	1.0	54.6
petB_p3	AAGAACCACAACGTCCTAGC	AGTCTTGTTACTCCCCTAAGTG	50.0	45.5	57.8	56.8	1600-1609 bp	1.0	54.5
petB_p4	GAACCACAACGTCCTAGCTT	AGTCTTGTTACTCCCCTAAGTG	50.0	45.5	57.8	56.8	1598-1607 bp	1.0	54.5
petB_p5	GAACCACAACGTCCTAGCTT	AGTCTTGTTACTCCCCTAAGTGA	50.0	43.5	57.8	58.3	1598-1607 bp	1.0	53.8