Hotspot

ndhF

SSC · 114255-116501 · 2247 bp

Back to Pouteria

Pi 0.00712

MarkerSeek score 46.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 24
pi: 0.00712
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGAAGCTTCTTATCTGGATGGG	GATTTGGTGAATCGAAAACA	45.5	35.0	57.6	52.4	2370-2377 bp	1.0	40.8
ndhF_p2	TGAAGCTTCTTATCTGGATGGG	GATTTGGTGAATCGAAAACAA	45.5	33.3	57.6	53.3	2370-2377 bp	1.0	40.8
ndhF_p3	GAAGCTTCTTATCTGGATGGGA	GATTTGGTGAATCGAAAACA	45.5	35.0	57.3	52.4	2369-2376 bp	1.0	40.8
ndhF_p4	AGCTTCTTATCTGGATGGGA	GATTTGGTGAATCGAAAACA	45.0	35.0	55.3	52.4	2367-2374 bp	1.0	40.8
ndhF_p5	GAAGCTTCTTATCTGGATGGGA	GATTTGGTGAATCGAAAACAA	45.5	33.3	57.3	53.3	2369-2376 bp	1.0	40.8