Hotspot

rpl20-rps12

LSC · 72607-73400 · 794 bp

Back to Pouteria

Pi 0.00462

MarkerSeek score 62.70

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 794
variable_sites: 5
pi: 0.00462
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	947-956 bp	1.0	75.9
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	946-955 bp	1.0	75.9
rpl20-rps12_p3	CTTGAATGAGCCCCTCGAAA	CAGCGTCGAGGAACTTGTAC	50.0	55.0	57.9	58.7	910-919 bp	1.0	74.9
rpl20-rps12_p4	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACTTGTAC	50.0	55.0	57.9	58.7	909-918 bp	1.0	74.9
rpl20-rps12_p5	TCCCCGTCTAATTCTGGTCA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.8	58.5	879-888 bp	1.0	74.9