Hotspot

psbF-psbE

LSC · 67922-67930 · 9 bp

Back to Pouteria

Pi 0.0741
MarkerSeek score 63.50
Primer available yes
Label psbF-psbE

Feature Metadata

label_name
psbF-psbE
feature_type
igs
region
LSC
length_bp
9
variable_sites
1
pi
0.0741
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbF-psbE_p1 GCCAACCATCGCACTGTAAA AACTGGCCGTTTTGATCCTT 50.0 45.0 59.1 58.0 111 bp 1.0 71.7
psbF-psbE_p2 GCCAACCATCGCACTGTAAA ACTGGCCGTTTTGATCCTTT 50.0 45.0 59.1 58.0 110 bp 1.0 71.7
psbF-psbE_p3 CCATCGCACTGTAAAAATGGG AACTGGCCGTTTTGATCCTT 47.6 45.0 57.9 58.0 106 bp 1.0 71.5
psbF-psbE_p4 CCATCGCACTGTAAAAATGGG ACTGGCCGTTTTGATCCTTT 47.6 45.0 57.9 58.0 105 bp 1.0 71.5
psbF-psbE_p5 GCCAACCATCGCACTGTAAA GAGCCGACAAGGAATTCCAT 50.0 50.0 59.1 58.0 135 bp 1.0 71.3