Hotspot

accD-psaI

LSC · 61504-62405 · 902 bp

Back to Pouteria

Pi 0.0107

MarkerSeek score 58.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 902
variable_sites: 14
pi: 0.0107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTTTCTTTCCTTTGAATCAA	AAGGCACAAAAATAGCGGGA	33.3	45.0	52.9	58.1	951-975 bp	1.0	45.2
accD-psaI_p2	GCTTTCTTTCCTTTGAATCAA	AGGCACAAAAATAGCGGGAA	33.3	45.0	52.9	58.1	950-974 bp	1.0	45.2
accD-psaI_p3	GCTTTCTTTCCTTTGAATCAA	CCATTGCAATTGCCGGAAAT	33.3	45.0	52.9	58.0	984-1008 bp	1.0	45.1
accD-psaI_p4	GCTTTCTTTCCTTTGAATCAA	AAGAAGCCATTGCAATTGCC	33.3	45.0	52.9	57.9	990-1014 bp	1.0	45.1
accD-psaI_p5	GCTTTCTTTCCTTTGAATCAAA	CCATTGCAATTGCCGGAAAT	31.8	45.0	53.8	58.0	984-1008 bp	1.0	45.1